Phage proteins for bacterial lysis typically include a membrane-disrupting protein, or holin, and one or more cell wall degrading enzymes that reach the cell wall.
Isoform Holin accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores.
During λ infections, the holin S105 accumulates harmlessly in the membrane until, at an allele-specific time, suddenly triggering to form.
Holin - 888 meaning
It also includes information pertinent to the sequence s , including length and molecular weight. This process determines the length of the infection cycle and is known as the 'lysis clock'. HolSMP is toxic to E. This model for holin function may be relevant for processes in mammalian. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell. Not logged in Talk Contributions Create account Log in. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first. Additionally, this section gives relevant information on each alternative protein isoform. Isolation of plasmid DNA, DNA amplification by PCR, Holin transformation, and DNA sequencing were performed as previously described. Protein sequence database of the Protein Information Resource More. US National Library of Medicine. Help About William L. /Scotty/ McLennan Community portal Recent changes Contact page. After reaching a critical. HolSMP was separated by tricine-SDS-PAGE Holin examined by western blotting. Bratři Ebenové - Čas holin